蛋白质组学

毕赤酵母表达从入门到精通

毕赤酵母表达从入门到提高

本文所涉及的技术要点主要来源于相关文献、网络和书籍,此外,一些操作中的技巧及经验等来自于本人及同学、朋友的总结,仅供大家参考,同时也请下载者不要传播尤其是以商业为目的的复制、传播等等;如有转载、传播请注明来源于“螺旋网”。若本文侵犯了您的版权或有任何不妥,请Email:kaosy@163.com告知。此外,由于学识、经验有限,本文难免会存在一些错误或缺陷,敬请不吝赐教,联系方式:kaosy@163.com同时因存在环境、试剂、仪器以及人为等原因,不能保证实验100%的成功,仅供参考。

写在前面,因为酵母里用到了很多遗传学的命名方法,所以建议大家首先看一下这个资料,可以让大家理解一些不常见的符号及意义。下载地址如下:http://www.helixnet.cn/bbs/thread-19215-1-1.htmL

我个人其实对于毕赤酵母表达系统掌握的比较一般,再加上个人的表达能力也并不出色,所以写出来的资料并不是十分好看。其实资料还是原装的好,我推荐大家多读几遍Invitrogen相关的技术手册。在这里,我把我手头的资料打包,方便大家,如果谁那有更好的资料,也欢迎大家补充。

1、  Pichia Expression Kit
2、pPIC9K-A Pichia Vector for MuLticopy Integration and Secreted Expression
3、Pichia Protocols
4、zeocin的信息
5、YNB配方
6、pGAPZA, B, and C-Pichia expression vectors for constitutive expressionand purification of recombinant proteins
7、毕赤酵母表达手册精译版中文版
8、Pichia Fermentation Process Guidelines
9、毕赤酵母表达实验手册中文版
10、pPICZα A, B, and C-Pichia expression vectors for selection on Zeocin. andpurification of secreted, recombinant proteins
11、Heterologous protein production using the Pichiapastoris expression system(英文综述)
12、Heterologous protein expression in the methylotrophic yeastPichia pastoris(英文综述)
13、A History of Research on Yeasts 1-Work by Chemists and biologists 1789-1850
14、A History of Research on Yeasts 2-louis pasteur and his contemporaries,1850-1880
15、A History of Research on Yeasts 3-emil fischer,eduard buchner and their contemporaries,1880-1900
16、A History of Research on Yeasts 4-cytology part II,1950-1990
17、A History of Research on Yeasts 5-the fermentation pathway
18、A History of Research on Yeasts 6-the main respiratory pathway
19、A History of Research on Yeasts 7-enzymic adaptation and reguLation
20、A History of Research on Yeasts 8-taxonomy
21、A history of research on yeasts 9-reguLation of sugar metabolism
22、A History of Research on Yeasts 10-foundations of yeast genetics
23、A history of research on yeasts 11-the study of solute transport-the first 90 years,simple and facilitated diffusion
24、A history of research on yeasts 12-medical yeasts part 1,Candida albicans
25、酵母的遗传学命名方法(相机拍照版)
以上资源下载地址:
http://d.namipan.com/d/%e8%9e%ba%e6%97%8b%e7%bd%91%e6%8e%a8%e8%8d%90.rar/f4e1456ad23d40630c412305610ad4c79ba2792208c73402

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