蛋白质组学

Mascot检索的问题

各位大侠,有个问题比较困惑,在Mascot上检索出来的蛋白并不是一一对应的,而是给出了几类蛋白,那么该如何选择呢?另外我的样本来自小鼠,如果检出的蛋白是(Rattus)的,是不是就不对呢?下面是一个例子,请各位百忙之中忙给予答复,谢谢。
1. gi|27691060 Mass: 16225 Score: 67 Expect: 0.025 Queries matched: 7
similar to tyrosine 3/tryptophan 5 -monooxygenase activation protein, zeta polypeptide; protein kinase C inhibitor protein-1; phospholipase A2; 14-3-3 zeta [Ra

--------------------------------------------------------------------------------
2. gi|200915 Mass: 10311 Score: 51 Expect: 0.88 Queries matched: 4
serum amyloid A (AA at 131)
gi|6755394 Mass: 13613 Score: 47 Expect: 2.3 Queries matched: 4
serum amyloid A 2 [Mus musculus]

--------------------------------------------------------------------------------
3. gi|34862239 Mass: 14818 Score: 48 Expect: 1.8 Queries matched: 5
hypothetical protein XP_347080 [Rattus norvegicus]

--------------------------------------------------------------------------------
4. gi|38085824 Mass: 65935 Score: 47 Expect: 2.4 Queries matched: 7
similar to KRAB zinc finger protein KR18 [Mus musculus]
gi|28486463 Mass: 16720 Score: 32 Expect: 67 Queries matched: 3
similar to brain otoferlin [Mus musculus]

--------------------------------------------------------------------------------
5. gi|34873635 Mass: 53305 Score: 43 Expect: 6.2 Queries matched: 5
similar to hypothetical protein FLJ22625 [Rattus norvegicus]

(0)

热评文章

发表评论